Intravital Imaging

We have extensively utilized the dorsal skinfold window chamber model (a.k.a. “backpack”) for development of appropriate biomaterial-based biomolecular delivery strategies for targeted recruitment of MP populations and for assessing their contributions to microvascular growth and remodeling. This chronic window chamber model permits both systemic and localized in vivo assessment of dynamic interactions of engineered materials with surrounding tissues in repeated measures during the initial 2-3 weeks after implantation. Cellular and molecular mechanisms involved in microvascular remodeling and the early inflammatory host response to pro-angiogenic stimulation can be assessed by means of in situ intravital fluorescence microscopy and end point flow cytometry analysis of implanted materials as well as tissue compartments in direct contact with or at various distances from the biomaterial. For a recent example of how these approaches may be applied, please see our recent paper.

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