See Our Latest Publication in Science Advances Focused on Specialized Pro-Angiogenic Neutrophils

To gain insights into neutrophil heterogeneity dynamics in the context of sterile inflammation and wound healing, we performed a pseudotime analysis of single-cell flow cytometry data using the spanning-tree progression analysis of density-normalized events algorithm. This enables us to view neutrophil transitional subsets along a pseudotime trajectory and identify distinct VEGFR1, VEGFR2, and CXCR4 high-expressing pro-angiogenic neutrophils. While the proresolving lipid mediator aspirin-triggered resolvin D1 (AT-RvD1) has a known ability to limit neutrophil infiltration, our analysis uncovers a mode of action in which AT-RvD1 leads to inflammation resolution through the selective reprogramming toward a therapeutic neutrophil subset. This accumulation leads to enhanced vascular remodeling in the skinfold window chamber and a proregenerative shift in macrophage and dendritic cell phenotype, resulting in improved wound closure after skin transplantation… [more]

Publication in JBMRA on Modulating S1P Receptor Signaling as a Regenerative Immunotherapy

Regeneration of skeletal muscle after volumetric injury is thought to be impaired by a dysregulated immune microenvironment that hinders endogenous repair mechanisms. Such defects result in fatty infiltration, tissue scarring, chronic inflammation, and debilitating functional deficits. Here, we evaluated the key cellular processes driving dysregulation in the injury niche through localized modulation of sphingosine‐1‐phosphate (S1P) receptor signaling. We employ dimensionality reduction and pseudotime analysis on single cell cytometry data to reveal heterogeneous immune cell subsets infiltrating preclinical muscle defects due to S1P receptor inhibition. We show that global knockout of S1P receptor 3 (S1PR3) is marked by an increase of muscle stem cells within injured tissue, a reduction in classically activated relative to alternatively activated macrophages, and increased bridging of regenerating myofibers across the defect. We found that local S1PR3 antagonism via nanofiber delivery of VPC01091 replicated key features of pseudotime immune cell recruitment dynamics… [more]

Check Out Our Latest Publication in Biomaterials

Inflammation after traumatic injury or surgical intervention is both a protective tissue response leading to regeneration and a potential cause of wound complications. One potentially successful strategy to harness to pro-regenerative roles of host inflammation is the localized delivery of bioactive materials to induce immune suppressive cellular responses by cells responding to injury. In this study, we designed a fully synthetic poly (ethylene) glycol (PEG)-based hydrogel to release the specialized pro-resolving lipid mediator aspirin-triggered resolvin-D1 (AT-RvD1) and recombinant human interleukin 10 (IL-10). We utilized a unique side-by-side internally controlled implant design wherein bioactive hydrogels… [more]

S’Dravious DeVeaux

S’Dravious “Dré” DeVeaux graduated from Georgia Southern University with a BS in Chemistry with a concentration in Biochemistry and minors in Biology and Mathematics in Fall 2015. Upon graduation he worked full-time as a Purification Chemist I before matriculating to Univeristy of Georgia (UGA) in Fall 2016. He graduate from UGA with his Masters in Biochemical Engineering in May 2018 with his thesis project titled, “Evaluation of Drug Delivery and Drug Testing Platforms for Breast Cancer Therapy.” He joined the Botchwey and Roy labs in Fall 2018. S’Dravious’s work is focused on lipidomic characterization of mesenchymal stromal/stem cells when cultured in 2D and in 3D hydrogel stem cell niches. He has thus performed targeted and untargeted mass spectrometry on MSCs while exploring the impact of demographics and culture parameters on their lipid metabolism. He plans on leveraging his experiences to investigate how modulating the sphingolipid pathway and biomaterial encapsulation can increase MSC immune potency for cell and cell-free manufacturing and therapeutics.

Favorite Atlanta Spot  – Petit Chou, restaurant with locally imported fresh vegetables and fruits.
Favorite TV show – How To Get Away With Murder
Fun Fact – I used to live in Ottawa, Ontario, Canada & I don’t like touching socks