CELL CHARACTERIZATION AND MANUFACTURING

We aim to develop new technologies to enhance the therapeutic potency of cell-based therapies. With support from the Marcus Center for Therapeutic Cell Characterization and Manufacturing (MC3M) we are utilizing high performance liquid chromatography-mass spectrometry (HPLC-MS/MS) to characterize the anti-inflammatory potential of mesenchymal stem cell (MSCs) based on metabolism of membrane sphingolipid (SLs). We use multi-variate analysis of lipidomic network profiles and cutting imaging analysis methods to gain mechanistic insight into the origin of observed changes in lipid biomarkers and SL signaling molecules. This analysis will lead to new technologies to predict, monitor, and adjust therapeutic cell potency during culture and expansion. We are also interested in optimizing new 3D biomaterials and bioreactors to specialize culture conditions for control of cell quality.

 

Cheryl San Emeterio, Ph.D.

Cheryl San Emeterio completed her undergraduate degree in biomedical engineering at Vanderbilt University. She joined Dr. Botchwey’s lab at Georgia Tech in 2012. Cheryl’s work focuses on engineering immunomodulatory biomaterials that harness pro-regenerative myeloid cell behavior during soft tissue injury. Cheryl’s recently published work demonstrates that blood-derived non-classical monocytes are biased progenitors of wound-healing macrophages within traumatic skin injuryCheryl expanded this knowledge into developing polymer scaffolds that release cues to enrich non-classical monocytes at sites of volumetric muscle loss, and showed that localization of pro-regenerative inflammation promotes muscle repair. In ongoing work, Cheryl is investigating the interplay of adaptive and innate immune cells within volumetric muscle defect healing by using immunomodulatory electrospun nanofiber scaffolds that tune lymphocyte and monocyte accumulation, and subsequently improve the quality of regenerated muscle tissue.